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Incubate the membrane for 20 min and then wash with DMF to take away uncoupled amino acids. Note that the facet chain has three methylene teams, so that though the terminal amino group shall be charged below physiological circumstances, the aspect chain does have significant hydrophobic character. Immunofluorescent staining of Human melanoma cells, utilizing Rabbit polyclonal to acetyl Lysine (ab21623) at 1:one hundred dilution.
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